Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.2003995
Title: Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus
Authors: Bergholt, M.S. 
Zheng, W. 
Ho, K.Y.
Yeoh, K.G.
Teh, M.
So, J.B.Y.
Huang, Z. 
Keywords: Barrett's esophagus
in vivo diagnosis
near-infrared Raman spectroscopy
Issue Date: 2013
Source: Bergholt, M.S., Zheng, W., Ho, K.Y., Yeoh, K.G., Teh, M., So, J.B.Y., Huang, Z. (2013). Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 8576 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.2003995
Abstract: Raman spectroscopy is a vibrational analytic technique sensitive to the changes in biomolecular composition and conformations occurring in tissue. With our most recent development of depth-resolved near-infrared (NIR) Raman endoscopy integrated with on-line diagnostic algorithms, in vivo real-time epithelial diagnostics has been realized under multimodal wide-field imaging (i.e., white- light reflectance (WLR), narrow-band imaging (NBI), autofluorescence imaging (AFI)) modalities. A selection of 43 patients who previously underwent Raman endoscopy (n=146 spectra) was used to render a robust model based on partial least squares - discriminant analysis (PLS-DA) for diagnosis of dysplasia in Barrett's esophagus. The Raman endoscopy technique was validated prospectively on 2 new esophageal patients for in vivo tissue diagnosis. The Raman endoscopic technique could identify esophageal high-grade dysplasia in vivo with an accuracy of 85.9% (sensitivity: 91.3% (21/23): specificity 83.3% (40/48)) on spectrum basis. This study realizes for the first time depth-resolved Raman endoscopy for real-time in vivo diagnosis of dysplasia in Barrett's epithelium at the biomolecular level. © 2013 Copyright SPIE.
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
URI: http://scholarbank.nus.edu.sg/handle/10635/74926
ISBN: 9780819493453
ISSN: 16057422
DOI: 10.1117/12.2003995
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

32
checked on Feb 16, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.