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https://doi.org/10.1117/12.2003995
Title: | Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus | Authors: | Bergholt, M.S. Zheng, W. Ho, K.Y. Yeoh, K.G. Teh, M. So, J.B.Y. Huang, Z. |
Keywords: | Barrett's esophagus in vivo diagnosis near-infrared Raman spectroscopy |
Issue Date: | 2013 | Citation: | Bergholt, M.S., Zheng, W., Ho, K.Y., Yeoh, K.G., Teh, M., So, J.B.Y., Huang, Z. (2013). Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 8576 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.2003995 | Abstract: | Raman spectroscopy is a vibrational analytic technique sensitive to the changes in biomolecular composition and conformations occurring in tissue. With our most recent development of depth-resolved near-infrared (NIR) Raman endoscopy integrated with on-line diagnostic algorithms, in vivo real-time epithelial diagnostics has been realized under multimodal wide-field imaging (i.e., white- light reflectance (WLR), narrow-band imaging (NBI), autofluorescence imaging (AFI)) modalities. A selection of 43 patients who previously underwent Raman endoscopy (n=146 spectra) was used to render a robust model based on partial least squares - discriminant analysis (PLS-DA) for diagnosis of dysplasia in Barrett's esophagus. The Raman endoscopy technique was validated prospectively on 2 new esophageal patients for in vivo tissue diagnosis. The Raman endoscopic technique could identify esophageal high-grade dysplasia in vivo with an accuracy of 85.9% (sensitivity: 91.3% (21/23): specificity 83.3% (40/48)) on spectrum basis. This study realizes for the first time depth-resolved Raman endoscopy for real-time in vivo diagnosis of dysplasia in Barrett's epithelium at the biomolecular level. © 2013 Copyright SPIE. | Source Title: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | URI: | http://scholarbank.nus.edu.sg/handle/10635/74926 | ISBN: | 9780819493453 | ISSN: | 16057422 | DOI: | 10.1117/12.2003995 |
Appears in Collections: | Staff Publications |
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