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|Title:||Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase|
|Keywords:||Malate dehydrogenase (MDH)|
Polyethylene glycol (PEG)
|Citation:||Nian, R., Kim, D.S., Tan, L., Kim, C.-W., Choe, W.-S. (2009-07). Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase. Biotechnology Progress 25 (4) : 1078-1085. ScholarBank@NUS Repository. https://doi.org/10.1002/btpr.175|
|Abstract:||The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperonemediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large-scale refolding processes. © 2009 American Institute of Chemical Engineers.|
|Source Title:||Biotechnology Progress|
|Appears in Collections:||Staff Publications|
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