Please use this identifier to cite or link to this item: https://doi.org/10.1002/btpr.175
Title: Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase
Authors: Nian, R.
Kim, D.S.
Tan, L.
Kim, C.-W.
Choe, W.-S. 
Keywords: Malate dehydrogenase (MDH)
Molecular bichaperone
Polyethylene glycol (PEG)
Protein refolding
Issue Date: Jul-2009
Source: Nian, R., Kim, D.S., Tan, L., Kim, C.-W., Choe, W.-S. (2009-07). Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase. Biotechnology Progress 25 (4) : 1078-1085. ScholarBank@NUS Repository. https://doi.org/10.1002/btpr.175
Abstract: The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperonemediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large-scale refolding processes. © 2009 American Institute of Chemical Engineers.
Source Title: Biotechnology Progress
URI: http://scholarbank.nus.edu.sg/handle/10635/74771
ISSN: 87567938
DOI: 10.1002/btpr.175
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