Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods

Abstract

This paper presents a new method for fabrication of biodegradable polymeric nanoparticles as carriers for anticancer drug paclitaxel. Emulsion solvent evaporation method was frequently employed to prepare polymeric nanoparticles for drug delivery systems in which the emulsifier such as PVA has to be used. In this study, direct dialysis was employed to fabricate paclitaxel-loaded biodegradable polymeric nanoparticles thus avoiding using emulsifier which potentially could remain on the surface of the nanoparticles and affect the biodegradability, biodistribution and drug-release behavior. The nanoparticles were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) for their morphology; laser scattering particle size analyzer for particle size distribution; and, zeta potential analyzer for their surface charge. The physical status of paclitaxel in polymer matrix by different theory drug loadings was studied by differential scanning calorimetry (DSC) and X-ray diffractometry pattern. The encapsulation efficiency and in vitro release profile were measured by high performance liquid chromatography (HPLC). C6 glioma cell line was used to evaluate the particle cellular uptake and cytotoxicity.