Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/73240
Title: Cell cycle synchronization of stem cells using inertial microfluidics
Authors: Lee, W.C.
Bhagat, A.A.S.
Huang, S.
Van Vliet, K.J.
Han, J.
Lim, C.T. 
Keywords: Cell cycle
Dean flows
Inertial forces
Size fractionation
Stem cells
Issue Date: 2010
Source: Lee, W.C.,Bhagat, A.A.S.,Huang, S.,Van Vliet, K.J.,Han, J.,Lim, C.T. (2010). Cell cycle synchronization of stem cells using inertial microfluidics. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 1 : 208-210. ScholarBank@NUS Repository.
Abstract: Cell cycle synchronization is of paramount importance for studying cellular properties and biological processes involved in various stages of the cell cycle. Here, we present a microfluidics based approach to synchronize the cell cycle of a primary - cells, human bone marrow-derived mesenchymal stem cells (hMSCs), using inertial forces in spiral microchan-nels. The device operating principle exploits the relationship between the volume (and thus diameter) of a cell and its phase in the cell cycle, in order to fractionate hMSCs populations into synchronized subpopulations enriched in cell cycle fractions of G0/G1, S and G2/M phases.
Source Title: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
URI: http://scholarbank.nus.edu.sg/handle/10635/73240
ISBN: 9781618390622
Appears in Collections:Staff Publications

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