Please use this identifier to cite or link to this item:
|Title:||Immobilization of flavin adenine dinucleotide (FAD) onto carbon cloth and its application as working electrode in an electroenzymatic bioreactor|
Flavin adenine dinucleotide
|Citation:||Jayabalan, R., Sathishkumar, M., Jeong, E.S., Mun, S.P., Yun, S.E. (2012-11). Immobilization of flavin adenine dinucleotide (FAD) onto carbon cloth and its application as working electrode in an electroenzymatic bioreactor. Bioresource Technology 123 : 686-689. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.07.045|
|Abstract:||A high porosity carbon cloth with immobilized FAD was employed as working electrode in electrochemical NADH-regeneration procedure. Carbon cloth was oxidized with hot acids to create surface carboxyl group and then coupled by adenine amino group of FAD with carbodiimide in the presence of N-hydroxysulfosuccinimide. The bioelectrocatalytic NADH-regeneration was coupled to the conversion of achiral substrate pyruvate into chiral product l-lactate by l-lactate dehydrogenase (l-LDH) within the same reactor. The conversion was completed at 96 h in bioreactor with FAD-modified carbon cloth, resulting in about 6. mM of l-lactate from 10. mM of pyruvate. While with bare carbon cloth, the yield at 120 h was around 5. mM. Immobilized FAD on the surface of carbon cloth electrode facilitated it to carry electrons from electrode to electron transfer enzymes; thereby NADH-regeneration was accelerated to drive the enzymatic reaction efficiently. © 2012 Elsevier Ltd.|
|Source Title:||Bioresource Technology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 12, 2018
WEB OF SCIENCETM
checked on Oct 3, 2018
checked on Oct 13, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.