Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.07.045
Title: Immobilization of flavin adenine dinucleotide (FAD) onto carbon cloth and its application as working electrode in an electroenzymatic bioreactor
Authors: Jayabalan, R.
Sathishkumar, M. 
Jeong, E.S.
Mun, S.P.
Yun, S.E.
Keywords: Bioreduction
Carbon cloth
Flavin adenine dinucleotide
Formate dehydrogenase
NADH regeneration
Issue Date: Nov-2012
Source: Jayabalan, R., Sathishkumar, M., Jeong, E.S., Mun, S.P., Yun, S.E. (2012-11). Immobilization of flavin adenine dinucleotide (FAD) onto carbon cloth and its application as working electrode in an electroenzymatic bioreactor. Bioresource Technology 123 : 686-689. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.07.045
Abstract: A high porosity carbon cloth with immobilized FAD was employed as working electrode in electrochemical NADH-regeneration procedure. Carbon cloth was oxidized with hot acids to create surface carboxyl group and then coupled by adenine amino group of FAD with carbodiimide in the presence of N-hydroxysulfosuccinimide. The bioelectrocatalytic NADH-regeneration was coupled to the conversion of achiral substrate pyruvate into chiral product l-lactate by l-lactate dehydrogenase (l-LDH) within the same reactor. The conversion was completed at 96 h in bioreactor with FAD-modified carbon cloth, resulting in about 6. mM of l-lactate from 10. mM of pyruvate. While with bare carbon cloth, the yield at 120 h was around 5. mM. Immobilized FAD on the surface of carbon cloth electrode facilitated it to carry electrons from electrode to electron transfer enzymes; thereby NADH-regeneration was accelerated to drive the enzymatic reaction efficiently. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/67770
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.07.045
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