Please use this identifier to cite or link to this item:
|Title:||A spatially addressable bead-based biosensor for simple and rapid DNA detection|
Single nucleotide polymorphism
|Citation:||Ng, J.K., Selamat, E.S., Liu, W.-T. (2008-01-18). A spatially addressable bead-based biosensor for simple and rapid DNA detection. Biosensors and Bioelectronics 23 (6) : 803-810. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2007.08.026|
|Abstract:||A simple glass-polymer-based biosensor that allows arrays of beads to be immobilized, separated and identified without any prior encoding is developed. To do so, distinct bead types that are conjugated with different oligonucleotide probes are sequentially spotted onto a polymeric matrix (or gel pad) on the surface of the device. The spotted beads are firmly immobilized to the gel pad, acquiring spatial codes that allow them to be identified. Throughput is enhanced by spotting different bead types onto hundreds of different gel pads. The bead-based biosensor was applied for the DNA-based detection of 10 model bacterial species and two single-nucleotide polymorphisms, and based on passive hybridization the final signals are obtained with single-mismatch specificity within 10 min. © 2007 Elsevier B.V. All rights reserved.|
|Source Title:||Biosensors and Bioelectronics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 18, 2018
WEB OF SCIENCETM
checked on Jun 4, 2018
checked on Jun 29, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.