Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.micron.2006.07.017
Title: The sampling limit in fluorescence microscopy
Authors: Heintzmann, R.
Sheppard, C.J.R. 
Keywords: Fluorescence microscopy
Imaging
Optical transfer function
Pixel size
Sampling
Issue Date: Feb-2007
Source: Heintzmann, R., Sheppard, C.J.R. (2007-02). The sampling limit in fluorescence microscopy. Micron 38 (2) : 145-149. ScholarBank@NUS Repository. https://doi.org/10.1016/j.micron.2006.07.017
Abstract: Sampling in fluorescence microscopy is treated using the concept of the three-dimensional (3D) optical transfer function (OTF). The border of the OTF frequency surface defines the required minimum sampling. The shape of the OTF is derived from simple considerations and valid for far-field high numerical aperture, vector theory. Optimal regular sampling is achieved by a hexagonal grid in 2D, and corresponding hexagonal structures, body-centered cubic (bcc) and hexagonal close-packed (hcp) structures, in 3D. As compared to standard (rectilinear grid) sampling a reduction of 13.4% in 2D and 29.3% in 3D can be achieved with optimized sampling. This reduction in data size is also accompanied by an imaging speed improvement, a reduction of sample bleaching, and can lead to imaging with better signal to noise ratio. © 2006 Elsevier Ltd. All rights reserved.
Source Title: Micron
URI: http://scholarbank.nus.edu.sg/handle/10635/67321
ISSN: 09684328
DOI: 10.1016/j.micron.2006.07.017
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