Please use this identifier to cite or link to this item: https://doi.org/10.1002/mabi.200900081
Title: Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells
Authors: Loh, X.J.
Gong, J.
Sakuragi, M.
Kitajima, T.
Liu, M.
Li, J. 
Ito, Y.
Keywords: Embryonic stem cell
Functional coating
Polyhydroxybutyric acid
Surface modification
Thermally responsive material
Issue Date: 10-Nov-2009
Source: Loh, X.J., Gong, J., Sakuragi, M., Kitajima, T., Liu, M., Li, J., Ito, Y. (2009-11-10). Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells. Macromolecular Bioscience 9 (11) : 1069-1079. ScholarBank@NUS Repository. https://doi.org/10.1002/mabi.200900081
Abstract: A thermoresponsive substrate based on a triblock copolymer, poly(N-isopropylacrylamide)-block-poly[(R)-3-hydroxybutyrate] -block-poly(N-isopropylacrylamide) (PNIPAAm-PHB-PNI-PAAm), co-coated with gelatin, was developed for the culture and non-enzymatic recovery of mouse embryonic stem cells. After culture, the cells could be detached by cooling at 4°C for 20 min without trypsin digestion. The embryonic stem cells remained undifferentiated after culture on the gelatin/copolymer-coated surfaces, similar to standard culture techniques. Overall, the triblock copolymer coating was superior to the PNI-PAAm homopolymer coating in terms of supporting better cell growth, being more stable, presenting a more homogeneous surface coating, and maintaining pluripotency of the embryonic stem cells. (Figure Presented) © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Source Title: Macromolecular Bioscience
URI: http://scholarbank.nus.edu.sg/handle/10635/67294
ISSN: 16165187
DOI: 10.1002/mabi.200900081
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