Please use this identifier to cite or link to this item: https://doi.org/10.1021/bm300936x
Title: Supramolecular anchoring of DNA polyplexes in cyclodextrin-based polypseudorotaxane hydrogels for sustained gene delivery
Authors: Li, Z.
Yin, H.
Zhang, Z.
Liu, K.L.
Li, J. 
Issue Date: 8-Oct-2012
Source: Li, Z., Yin, H., Zhang, Z., Liu, K.L., Li, J. (2012-10-08). Supramolecular anchoring of DNA polyplexes in cyclodextrin-based polypseudorotaxane hydrogels for sustained gene delivery. Biomacromolecules 13 (10) : 3162-3172. ScholarBank@NUS Repository. https://doi.org/10.1021/bm300936x
Abstract: A cyclodextrin-based supramolecular hydrogel system with supramolecularly anchored active cationic copolymer/plasmid DNA (pDNA) polyplexes was studied as a sustained gene delivery carrier. A few biodegradable triblock copolymers of methoxy-poly(ethylene glycol)-b-poly(ε-caprolactone)-b-poly[2- (dimethylamino)ethyl methacrylate] (MPEG-PCL-PDMAEMA) with well-defined cationic block lengths were prepared to condense pDNA. The MPEG-PCL-PDMAEMA copolymers exhibit good ability to condense pDNA into 275-405 nm polyplexes with hydrophilic MPEG in the outer corona. The MPEG corona imparted greater stability to the pDNA polyplexes and also served as an anchoring segment when the pDNA polyplexes were encapsulated in α-CD-based supramolecular polypseudorotaxane hydrogels. More interestingly, the resultant hydrogels were able to sustain release of pDNA up to 6 days. The pDNA was released in the form of polyplex nanoparticles as it was bound electrostatically to the cationic segment of the MPEG-PCL-PDMAEMA copolymers. The bioactivity of the released pDNA polyplexes at various durations was further investigated. Protein expression level of pDNA polyplexes released over the durations was comparable to that of freshly prepared PEI polyplexes. Being thixotropic and easily prepared without using organic solvent, this supramolecular in situ gelling system has immense potential as an injectable carrier for sustained gene delivery. © 2012 American Chemical Society.
Source Title: Biomacromolecules
URI: http://scholarbank.nus.edu.sg/handle/10635/67289
ISSN: 15257797
DOI: 10.1021/bm300936x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

71
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

69
checked on Nov 22, 2017

Page view(s)

39
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.