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Title: Scalable cell alignment on optical media substrates
Authors: Anene-Nzelu, C.G.
Choudhury, D.
Li, H.
Fraiszudeen, A.
Peh, K.-Y.
Toh, Y.C.
Ng, S.H.
Leo, H.L. 
Yu, H. 
Keywords: Alignment
Grooved substrates
Optical gratings
Optical media
Issue Date: Jul-2013
Citation: Anene-Nzelu, C.G., Choudhury, D., Li, H., Fraiszudeen, A., Peh, K.-Y., Toh, Y.C., Ng, S.H., Leo, H.L., Yu, H. (2013-07). Scalable cell alignment on optical media substrates. Biomaterials 34 (21) : 5078-5087. ScholarBank@NUS Repository.
Abstract: Cell alignment by underlying topographical cues has been shown to affect important biological processes such as differentiation and functional maturation invitro. However, the routine use of cell culture substrates with micro- or nano-topographies, such as grooves, is currently hampered by the high cost and specialized facilities required to produce these substrates. Here we present cost-effective commercially available optical media as substrates for aligning cells in culture. These optical media, including CD-R, DVD-R and optical grating, allow different cell types to attach and grow well on them. The physical dimension of the grooves in these optical media allowed cells to be aligned in confluent cell culture with maximal cell-cell interaction and these cell alignment affect the morphology and differentiation of cardiac (H9C2), skeletal muscle (C2C12) and neuronal (PC12) cell lines. The optical media is amenable to various chemical modifications with fibronectin, laminin and gelatin for culturing different cell types. These low-cost commercially available optical media can serve as scalable substrates for research or drug safety screening applications in industry scales. © 2013 Elsevier Ltd.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2013.03.070
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