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|Title:||Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds|
|Citation:||Salgado, A.J., Gomes, M.E., Chou, A., Coutinho, O.P., Reis, R.L., Hutmacher, D.W. (2002-05-31). Preliminary study on the adhesion and proliferation of human osteoblasts on starch-based scaffolds. Materials Science and Engineering C 20 (1-2) : 27-33. ScholarBank@NUS Repository. https://doi.org/10.1016/S0928-4931(02)00009-7|
|Abstract:||Up to today, several techniques have been used to produce biodegradable porous scaffolds for tissue engineering. In this work, a new technique based on extrusion by using blowing agents in combination with a 50:50 (wt.%) blend of starch/cellulose acetate (SCA) was studied. The results show that by using this technique it was possible to obtain scaffolds with 70% of porosity and a fully interconnected network of pores, with sizes ranging from 200 to 500 μm. After their production, the mechanical properties of these scaffolds were tested, presenting a compressive modulus of 124.6 ± 27.2 MPa and a compressive strength of 8.0 ± 0.9 MPa. These values are within the best found in the literature and show that by using this technique, it is possible to produce scaffolds that, from a mechanical standpoint, may be suitable for bone tissue engineering. Cell culturing experiments showed that cells were viable and that there were no signs of cellular death after 3 weeks of culture. Finally, biochemical assays demonstrate that cells maintained the osteogenic phenotype throughout the experiment and deposition of mineralized extracellular matrix could be detected. © 2002 Elsevier Science B.V. All rights reserved.|
|Source Title:||Materials Science and Engineering C|
|Appears in Collections:||Staff Publications|
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