Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.tea.2011.0580
Title: Osteochondral interface generation by rabbit bone marrow stromal cells and osteoblasts coculture
Authors: Chen, K.
Teh, T.K.H. 
Ravi, S.
Toh, S.L. 
Goh, J.C.H. 
Issue Date: 1-Sep-2012
Source: Chen, K., Teh, T.K.H., Ravi, S., Toh, S.L., Goh, J.C.H. (2012-09-01). Osteochondral interface generation by rabbit bone marrow stromal cells and osteoblasts coculture. Tissue Engineering - Part A 18 (17-18) : 1902-1911. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tea.2011.0580
Abstract: Physiological osteochondral interface regeneration is a significant challenge. This study aims to investigate the effect of the coculture of chondrogenic rabbit bone marrow stromal cells (rBMSCs) with rabbit osteoblasts in a specially designed two-dimensional (2D)-three-dimensional (3D) co-interface culture to develop the intermediate osteochondral region in vitro. The 2D-3D coculture system was set up by first independently culturing chondrogenic rBMSCs on a scaffold and osteoblasts in cell culture plates, and subsequently placed in contact and cocultured. As control, samples not cocultured with osteoblasts were used. The regulatory effects exerted by osteoblasts on chondrogenic rBMSCs were quantified by real-time polymerase chain reaction. To study the effect of coculture on cells located in different parts of the scaffold, samples were separated into two parts and significantly different gene expression patterns were found between them. In comparison with the control group, a significant moderate downregulation of chondrogenic marker genes, such as Collagen II and Aggrecan was observed. However, the Sox-9 and Collagen I expression increased. More importantly, chondrogenic rBMSCs in the coculture system were shown to form the osteochondral interface layer by expressing calcified cartilage zone specific extracellular matrix marker Collagen X and the hypertrophic chondrocyte marker MMP-13, which were not observed in the control group. Specifically, only the chondrogenic rBMSC layer in contact with the osteoblasts expressed Collagen X and MMP-13, indicating the positive influence of the coculture upon interface formation. Biochemical analyses, histology results, and immunohistochemical staining further supported this observation. In conclusion, this study revealed that specific regulatory stimulations from osteoblasts in the 2D-3D interface coculture system could induce the formation of ostochondral interface for the purpose of osteochondral tissue engineering. © Copyright 2012, Mary Ann Liebert, Inc.
Source Title: Tissue Engineering - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/67205
ISSN: 19373341
DOI: 10.1089/ten.tea.2011.0580
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