Please use this identifier to cite or link to this item: https://doi.org/10.1039/c2an16123k
Title: On-site chemical reaction lights up protein assemblies in cells
Authors: Win, K.Y.
Teng, C.P.
Bee Jin, M.L.
Ye, E.
Tansil, N.C.
Han, M.-Y. 
Issue Date: 21-May-2012
Source: Win, K.Y., Teng, C.P., Bee Jin, M.L., Ye, E., Tansil, N.C., Han, M.-Y. (2012-05-21). On-site chemical reaction lights up protein assemblies in cells. Analyst 137 (10) : 2328-2332. ScholarBank@NUS Repository. https://doi.org/10.1039/c2an16123k
Abstract: Here we report a fast and effective method to visualize interactive proteins across intact mammalian cells via on-site formation of fluorescence using instant reaction of non-fluorescent fluorescamine with primary amines on proteins. Without interference by fluorescence background, this fluorogenic labelling opens a way for selective identification of primary amine-rich interacting proteins, efficient mapping and real-time monitoring of their spatial distribution in assemblies/network, and fast differentiation of cellular types. Without adverse effect on biological functions, this labelling method also provides new insights to comprehend important aspects of cellular functions of organelles and their relation to health imperfections for disease diagnostics and imaging-guided therapy. © 2012 The Royal Society of Chemistry.
Source Title: Analyst
URI: http://scholarbank.nus.edu.sg/handle/10635/67200
ISSN: 00032654
DOI: 10.1039/c2an16123k
Appears in Collections:Staff Publications

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