Please use this identifier to cite or link to this item: https://doi.org/10.1039/b904171k
Title: Novel poly(N-isopropylacrylamide)-poly[(R)-3-hydroxybutyrate]-poly(N- isopropylacrylamide) triblock copolymer surface as a culture substrate for human mesenchymal stem cells
Authors: Loh, X.J.
Cheong, W.C.D.
Li, J. 
Ito, Y.
Issue Date: 2009
Citation: Loh, X.J., Cheong, W.C.D., Li, J., Ito, Y. (2009). Novel poly(N-isopropylacrylamide)-poly[(R)-3-hydroxybutyrate]-poly(N- isopropylacrylamide) triblock copolymer surface as a culture substrate for human mesenchymal stem cells. Soft Matter 5 (15) : 2937-2946. ScholarBank@NUS Repository. https://doi.org/10.1039/b904171k
Abstract: A thermoresponsive surface was fabricated by coating a substrate with a thermoresponsive amphiphilic triblock copolymer, poly(N-isopropylacrylamide)- poly[(R)-3-hydroxybutyrate]-poly(N-isopropylacrylamide) (PNIPAAm-PHB-PNIPAAm), and used for the attachment and nonenzymatic temperature-induced detachment of human mesenchymal stem cells. The copolymer self-assembled into micelles in water and formed stable attachments to the substrate by hydrophobic interactions between the micelle core and the substrate surface. The stability of the polymer attachment was confirmed by ATR-FTIR analysis of the polymer-coated substrates before and after soaking in water. Illustration of the copolymer coating on the cell culture substrate was accomplished by applying Farago's implicit-solvent model for studying the self-assembly of large molecules. The copolymer coating enhanced the proliferation of human mesenchymal stem cells compared with either PNIPAAm homopolymer-coated or noncoated surface. The copolymer-coated substrate showed a change in the surface hydrophilicity when the temperature was changed. After a period of culture, the cells could be detached by cooling at 4 °C for 20 min without trypsin treatment. © The Royal Society of Chemistry 2009.
Source Title: Soft Matter
URI: http://scholarbank.nus.edu.sg/handle/10635/67198
ISSN: 1744683X
DOI: 10.1039/b904171k
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