Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0037904
Title: Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior
Authors: Zeiger, A.S.
Loe, F.C. 
Li, R.
Raghunath, M. 
van Vliet, K.J.
Issue Date: 23-May-2012
Source: Zeiger, A.S., Loe, F.C., Li, R., Raghunath, M., van Vliet, K.J. (2012-05-23). Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior. PLoS ONE 7 (5) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0037904
Abstract: Microenvironments of biological cells are dominated in vivo by macromolecular crowding and resultant excluded volume effects. This feature is absent in dilute in vitro cell culture. Here, we induced macromolecular crowding in vitro by using synthetic macromolecular globules of nm-scale radius at physiological levels of fractional volume occupancy. We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. Macromolecular crowding can thus aid the design of more physiologically relevant in vitro studies and devices for MSCs and other cells, by increasing the fidelity between materials synthesized by cells in vivo and in vitro. © 2012 Zeiger et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/67147
ISSN: 19326203
DOI: 10.1371/journal.pone.0037904
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

31
checked on Dec 11, 2017

WEB OF SCIENCETM
Citations

25
checked on Dec 11, 2017

Page view(s)

24
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.