Please use this identifier to cite or link to this item: https://doi.org/10.1039/b806907g
Title: In-situ measurement of cellular microenvironments in a microfluidic device
Authors: Lin, Z.
Cherng-Wen, T.
Roy, P. 
Trau, D. 
Issue Date: 2009
Source: Lin, Z., Cherng-Wen, T., Roy, P., Trau, D. (2009). In-situ measurement of cellular microenvironments in a microfluidic device. Lab on a Chip - Miniaturisation for Chemistry and Biology 9 (2) : 257-262. ScholarBank@NUS Repository. https://doi.org/10.1039/b806907g
Abstract: We report on the integration of optical microsensors into a cell culture microchannel device. We demonstrate the possibility of measuring the glucose and oxygen concentrations in the microenvironment of the mammalian cells cultured in a microchannel device. Furthermore, cell proliferation and morphology could be monitored microscopically while these measurements were being made. Through the use of multiple sensors along the length of the microchannel, concentration gradients of various metabolites, such as oxygen, as well as the effects of cell uptake and perfusion rate of growth medium on these gradients could be studied. As such, the system allowed real-time observations of the cells' response to their chemical microenvironment. Our approach allows cell culture and cell assays to be performed simultaneously in an integrated microchannel system with potential applications as a research tool or drug screening method. © 2009 The Royal Society of Chemistry.
Source Title: Lab on a Chip - Miniaturisation for Chemistry and Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/67117
ISSN: 14730197
DOI: 10.1039/b806907g
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