Please use this identifier to cite or link to this item: https://doi.org/10.1364/BOE.1.001026
Title: High-speed focal modulation microscopy using acousto-optical modulators
Authors: Chong, S.P.
Wong, C.H.
Wong, K.F.
Sheppard, C.J.R. 
Chen, N. 
Issue Date: 1-Oct-2010
Source: Chong, S.P.,Wong, C.H.,Wong, K.F.,Sheppard, C.J.R.,Chen, N. (2010-10-01). High-speed focal modulation microscopy using acousto-optical modulators. Biomedical Optics Express 1 (3) : 1026-1033. ScholarBank@NUS Repository. https://doi.org/10.1364/BOE.1.001026
Abstract: Focal Modulation Microscopy (FMM) is a single-photon excitation fluorescence microscopy technique which effectively rejects the out-of-focus fluorescence background that arises when imaging deep inside biological tissues. Here, we report on the implementation of FMM in which laser intensity modulation at the focal plane is achieved using acousto-optic modulators (AOM). The modulation speed is greatly enhanced to the MHz range and thus enables real-time image acquisition. The capability of FMM is demonstrated by imaging fluorescence labeled vasculatures in mouse brain as well as self-made tissue phantom. © 2010 Optical Society of America.
Source Title: Biomedical Optics Express
URI: http://scholarbank.nus.edu.sg/handle/10635/67084
ISSN: 21567085
DOI: 10.1364/BOE.1.001026
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