Please use this identifier to cite or link to this item: https://doi.org/10.1364/AO.49.000752
Title: Divided-aperture technique for fluorescence confocal microscopy through scattering media
Authors: Gong, W. 
Si, K.
Sheppard, C.J.R. 
Issue Date: 1-Feb-2010
Source: Gong, W., Si, K., Sheppard, C.J.R. (2010-02-01). Divided-aperture technique for fluorescence confocal microscopy through scattering media. Applied Optics 49 (4) : 752-757. ScholarBank@NUS Repository. https://doi.org/10.1364/AO.49.000752
Abstract: We present a diffraction analysis for image formation in fluorescence confocal microscopy with divided apertures. The three-dimensional optical transform function is given, and axial resolution and transverse resolution are investigated. The results show that the employment of a divided-aperture technique in fluorescence confocal microscopy can enhance the rejection of background scattering. In addition, the axial resolution and transverse resolution can be improved by adjusting the width of the divider strip. For a given detector size, an optimum value of the divider strip width is given to obtain the best axial resolution or transverse resolution. The integrated intensity and signal-to-background ratio are also discussed. © 2010 Optical Society of America.
Source Title: Applied Optics
URI: http://scholarbank.nus.edu.sg/handle/10635/67007
ISSN: 00036935
DOI: 10.1364/AO.49.000752
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