Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ab.2010.11.031
Title: Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis
Authors: Cheng, J. 
Yim, O.-S.
Low, P.-S.
Tay, S.K.H.
Yap, E.P.H.
Lai, P.-S.
Issue Date: 1-Mar-2011
Source: Cheng, J., Yim, O.-S., Low, P.-S., Tay, S.K.H., Yap, E.P.H., Lai, P.-S. (2011-03-01). Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis. Analytical Biochemistry 410 (1) : 158-160. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2010.11.031
Abstract: Heteroduplex formation, required for the complete detection of hemi/homozygotes using high-resolution melting analysis, can be induced either by pre-PCR mixing of genomic DNAs or by post-PCR mixing of PCR products from unknown and reference samples. This study investigates the effects of both methods using two single nucleotide polymorphisms in X-linked DMD gene. The results show that both methods resulted in the same effect when mixing samples with the same gene copy number. Mixing samples with different gene copy numbers has not been previously explored and we show that post-PCR mixing is insensitive to gene copy number differences as compared to pre-PCR mixing. © 2010 Elsevier Inc. All rights reserved.
Source Title: Analytical Biochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/66998
ISSN: 00032697
DOI: 10.1016/j.ab.2010.11.031
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