Please use this identifier to cite or link to this item:
|Title:||Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis|
|Authors:||Cheng, J. |
|Source:||Cheng, J., Yim, O.-S., Low, P.-S., Tay, S.K.H., Yap, E.P.H., Lai, P.-S. (2011-03-01). Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis. Analytical Biochemistry 410 (1) : 158-160. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2010.11.031|
|Abstract:||Heteroduplex formation, required for the complete detection of hemi/homozygotes using high-resolution melting analysis, can be induced either by pre-PCR mixing of genomic DNAs or by post-PCR mixing of PCR products from unknown and reference samples. This study investigates the effects of both methods using two single nucleotide polymorphisms in X-linked DMD gene. The results show that both methods resulted in the same effect when mixing samples with the same gene copy number. Mixing samples with different gene copy numbers has not been previously explored and we show that post-PCR mixing is insensitive to gene copy number differences as compared to pre-PCR mixing. © 2010 Elsevier Inc. All rights reserved.|
|Source Title:||Analytical Biochemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 21, 2018
WEB OF SCIENCETM
checked on Jan 17, 2018
checked on Feb 19, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.