Kinetic and equilibrium study of the enantioseparation of fluoxetine on a new β-cyclodextrin column by high performance liquid chromatography

Abstract

A new β-cyclodextrin preparative chiral stationary phase was developed and utilized to separate racemic fluoxetine by high performance liquid chromatography. Kinetic and equilibrium constants for the chromatographic enantioseparation of racemic fluoxetine, using water and buffer (TTAA) as the mobile phase, were evaluated by moment analysis on the basis of equilibrium-dispersive and linear driving force models. Other parameters, including the voidage of the column and the axial dispersion coefficient, were also determined. The parameters obtained were used in the simulation of two single enantiomer band profiles. It was found that the experimental and simulated results match quite well confirming that the parameters used in simulation were correct.