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|Title:||Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB|
|Authors:||Paul Chen, J. |
|Source:||Paul Chen, J.,Wu, S. (2000-09). Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB. Journal of Chemical Technology and Biotechnology 75 (9) : 798-802. ScholarBank@NUS Repository. https://doi.org/10.1002/1097-4660(200009)75:9<798|
|Abstract:||A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.|
|Source Title:||Journal of Chemical Technology and Biotechnology|
|Appears in Collections:||Staff Publications|
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