Please use this identifier to cite or link to this item: https://doi.org/10.1128/AEM.71.10.6453-6457.2005
Title: Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu, W.-T. 
Wu, J.-H. 
Li, E.S.-Y.
Selamat, E.S.
Issue Date: Oct-2005
Source: Liu, W.-T., Wu, J.-H., Li, E.S.-Y., Selamat, E.S. (2005-10). Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies. Applied and Environmental Microbiology 71 (10) : 6453-6457. ScholarBank@NUS Repository. https://doi.org/10.1128/AEM.71.10.6453-6457.2005
Abstract: The effects of temperature, salt concentration, and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The emission intensities of different fluorophores without hybridization were observed to vary, each to a different extent, to mainly temperature changes. Rhodamine red, TAMRA (tetramethylrhodamine), and dyes from the carbocyanide group exhibited the largest variations, and Texas Red and Oregon Green exhibited the smallest variations. This temperature dependency was shown to affect results obtained during melting curve analysis in DNA microarray studies. To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Source Title: Applied and Environmental Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/65526
ISSN: 00992240
DOI: 10.1128/AEM.71.10.6453-6457.2005
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