Please use this identifier to cite or link to this item: https://doi.org/10.1002/asia.200600257
Title: Tetrahydrofuran activates fluorescence resonant energy transfer from a cationic conjugated polyelectrolyte to fluorescein-labeled DNA in aqueous media
Authors: Liu, B. 
Bazan, G.C.
Keywords: Chromophores
DNA
Electron transfer
Fluorescence
FRET (fluorescence resonant energy transfer)
Issue Date: 2007
Source: Liu, B., Bazan, G.C. (2007). Tetrahydrofuran activates fluorescence resonant energy transfer from a cationic conjugated polyelectrolyte to fluorescein-labeled DNA in aqueous media. Chemistry - An Asian Journal 2 (4) : 499-504. ScholarBank@NUS Repository. https://doi.org/10.1002/asia.200600257
Abstract: A cationic water-soluble conjugated polyelectrolyte, poly[9,9-bis(6″- (N,N,N-trimethylammonium)hexyl)-fluorene-co-alt-2,5-bis(6′-(N,N, N-trimethylammonium)hexyloxyphenylene) tetrabromide], was synthesized. Fluorescence resonant energy transfer (FRET) experiments between the polymer and fluorescein-labeled single-stranded DNA (ssDNA-Fl) were conducted in aqueous buffer and THF/ buffer mixtures. Weak fluorescence emission in aqueous buffer was observed upon excitation of the polymer, whereas addition of THF turned on the fluorescence. Fluorescence self-quenching of ssDNA-Fl in the ssDNA-Fl/polymer complexes as well as electron transfer from the polymer to fluorescein may account for the low fluorescence emission in buffer. The improved sensitization of fluorescence by the polymer observed in THF/buffer could be attributed to the weaker binding between the polymer and ssDNA-Fl and a decrease in dielectric constant of the solvent mixture, which disfavors electron transfer. THF-assisted signal sensitization was also observed for the polymer and fluorescein-labeled double-stranded DNA (dsDNA-Fl). These results indicate that the use of cosolvent provides a strategy to improve the detection sensitivity for biosensors based on the optical amplification provided by conjugated polymers. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
Source Title: Chemistry - An Asian Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/64693
ISSN: 18614728
DOI: 10.1002/asia.200600257
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