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Title: Self-assembled biodegradable nanoparticles developed by direct dialysis for the delivery of paclitaxel
Authors: Xie, J. 
Wang, C.-H. 
Keywords: Cellular uptake
Issue Date: Dec-2005
Citation: Xie, J., Wang, C.-H. (2005-12). Self-assembled biodegradable nanoparticles developed by direct dialysis for the delivery of paclitaxel. Pharmaceutical Research 22 (12) : 2079-2090. ScholarBank@NUS Repository.
Abstract: Purpose. The main objective of this study was to obtain self-assembled biodegradable nanoparticles by a direct dialysis method for the delivery of anticancer drug. The in vitro cellular particle uptake and cytotoxicity to C6 glioma cell line were investigated. Methods. Self-assembled anticancer drugs-paclitaxel-loaded poly(d,l-lactic-co-glycolic acid) (PLGA) and poly(l-lactic acid) (PLA) nanoparticles-were achieved by direct dialysis. The physical and chemical properties of nanoparticles were characterized by various state-of-the-art techniques. The encapsulation efficiency and in vitro release profile were measured by high-performance liquid chromatography. Particle cellular uptake was studied using confocal microscopy, microplate reader, and flow cytometry. In addition, the cytotoxicity of this drug delivery system was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on C6 glioma cell line to predict the possible dose response of paclitaxel-loaded PLGA and PLA nanoparticles. Results. PLGA and PLA nanoparticles with or without vitamin E tocopherol polyethylene glycol succinate (TPGS) as an additive were obtained, in which the sustained release of paclitaxel of more than 20 days was achieved. The coumarin6-loaded PLGA and PLA nanoparticles could penetrate the C6 glioma cell membrane and be internalized. The cytotoxicity of paclitaxel-loaded nanoparticles seemed to be higher than that of commercial Taxol® after 3 days incubation when paclitaxel concentrations were 10 and 20 μg/ml. Conclusions. Direct dialysis could be employed to achieve paclitaxel-loaded PLGA and PLA nanoparticles, which could be internalized by C6 glioma cells and enhance the cytotoxicity of paclitaxel because of its penetration to the cytoplasm and sustained release property. © 2005 Springer Science + Business Media, Inc.
Source Title: Pharmaceutical Research
ISSN: 07248741
DOI: 10.1007/s11095-005-7782-y
Appears in Collections:Staff Publications

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