Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00253-008-1728-3
Title: Induction of ortho- and meta-cleavage pathways in Pseudomonas in biodegradation of high benzoate concentration: MS identification of catabolic enzymes
Authors: Cao, B.
Geng, A.
Loh, K.-C. 
Keywords: 2-DE
Benzoate biodegradation
MALDI-TOF MS
Meta-cleavage pathway
Ortho-cleavage pathway
Pseudomonas putida
Issue Date: Nov-2008
Source: Cao, B., Geng, A., Loh, K.-C. (2008-11). Induction of ortho- and meta-cleavage pathways in Pseudomonas in biodegradation of high benzoate concentration: MS identification of catabolic enzymes. Applied Microbiology and Biotechnology 81 (1) : 99-107. ScholarBank@NUS Repository. https://doi.org/10.1007/s00253-008-1728-3
Abstract: The degradation pathways of benzoate at high concentration in Pseudomonas putida P8 were directly elucidated through mass spectrometric identification of some key catabolic enzymes. Proteins from P. putida P8 grown on benzoate or succinate were separated using two-dimensional gel electrophoresis. For cells grown on benzoate, eight distinct proteins, which were absent in the reference gel patterns from succinate-grown cells, were found. All the eight proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as catabolic enzymes involved in benzoate degradation. Among them, CatB (EC5.5.1.1), PcaI (EC2.8.3.6), and PcaF (EC2.3.1.174) were the enzymes involved in the ortho-cleavage pathway; DmpC (EC1.2.1.32), DmpD (EC3.1.1.-), DmpE (EC4.2.1.80), DmpF (EC1.2.1.10), and DmpG (EC4.1.3.-) were the meta-cleavage pathway enzymes. In addition, enzyme activity assays showed that the activities of both catechol 1,2-dioxygenase (C12D; EC1.13.11.1) and catechol 2,3-dioxygenase (C23D; EC1.13.11.2) were detected in benzoate-grown P. putida cells, undoubtedly suggesting the simultaneous expression of both the ortho- and the meta-cleavage pathways in P. putida P8 during the biodegradation of benzoate at high concentration. © 2008 Springer-Verlag.
Source Title: Applied Microbiology and Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/64094
ISSN: 01757598
DOI: 10.1007/s00253-008-1728-3
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