Please use this identifier to cite or link to this item:
Title: Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification
Authors: Ye, L.
Zhao, H.
Li, Z. 
Wu, J.C.
Keywords: Acid tolerance
Error-prone amplification
Lactic acid
Lactobacillus pentosus
Whole genome
Issue Date: May-2013
Citation: Ye, L., Zhao, H., Li, Z., Wu, J.C. (2013-05). Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification. Bioresource Technology 135 : 459-463. ScholarBank@NUS Repository.
Abstract: Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20. g/L of glucose to produce lactic acid at a yield of 95% in 1. L MRS medium at pH 3.8 within 36. h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.10.042
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 19, 2019


checked on Feb 11, 2019

Page view(s)

checked on Feb 9, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.