Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.10.042
Title: Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification
Authors: Ye, L.
Zhao, H.
Li, Z. 
Wu, J.C.
Keywords: Acid tolerance
Error-prone amplification
Lactic acid
Lactobacillus pentosus
Whole genome
Issue Date: May-2013
Source: Ye, L.,Zhao, H.,Li, Z.,Wu, J.C. (2013-05). Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification. Bioresource Technology 135 : 459-463. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.10.042
Abstract: Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20. g/L of glucose to produce lactic acid at a yield of 95% in 1. L MRS medium at pH 3.8 within 36. h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/64066
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.10.042
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