Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10616-004-6173-2
Title: Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures
Authors: Wong, V.V.T.
Ho, K.W.
Yap, M.G.S. 
Keywords: Insulin
Mammalian cell culture
Protein-free medium
Trace metals
Zinc
Issue Date: 2004
Source: Wong, V.V.T., Ho, K.W., Yap, M.G.S. (2004). Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures. Cytotechnology 45 (3) : 107-115. ScholarBank@NUS Repository. https://doi.org/10.1007/s10616-004-6173-2
Abstract: Insulin is involved in a number of cellular functions, including the stimulation of cell growth, cell cycle progression and glucose uptake and is a common protein supplement in serum-free mammalian cell culture media. However, several trace metals have previously been reported to exhibit insulin-like effects on specific cell types. As a step towards developing chemically-defined, protein-free media for mammalian cells, we tested the effectiveness of five trace metals (cadmium, nickel, lithium, vanadium and zinc) as a replacement for insulin. Four cell lines of biotechnological relevance were used, including the hybridoma CRL1606, the myeloma NS0, and the Chinese hamster ovary cell lines CHO-IFN and CHO-K1. Zinc was found to be an effective insulin replacement for the hybridoma, myeloma and CHO-K1 cells. Cell growth, cell cycle progression and antibody production was not affected by the substitution. Furthermore, no adaptation procedure was required. © 2004 Kluwer Academic Publishers.
Source Title: Cytotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/63874
ISSN: 09209069
DOI: 10.1007/s10616-004-6173-2
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