Please use this identifier to cite or link to this item:
Title: Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures
Authors: Wong, V.V.T.
Ho, K.W.
Yap, M.G.S. 
Keywords: Insulin
Mammalian cell culture
Protein-free medium
Trace metals
Issue Date: 2004
Citation: Wong, V.V.T., Ho, K.W., Yap, M.G.S. (2004). Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures. Cytotechnology 45 (3) : 107-115. ScholarBank@NUS Repository.
Abstract: Insulin is involved in a number of cellular functions, including the stimulation of cell growth, cell cycle progression and glucose uptake and is a common protein supplement in serum-free mammalian cell culture media. However, several trace metals have previously been reported to exhibit insulin-like effects on specific cell types. As a step towards developing chemically-defined, protein-free media for mammalian cells, we tested the effectiveness of five trace metals (cadmium, nickel, lithium, vanadium and zinc) as a replacement for insulin. Four cell lines of biotechnological relevance were used, including the hybridoma CRL1606, the myeloma NS0, and the Chinese hamster ovary cell lines CHO-IFN and CHO-K1. Zinc was found to be an effective insulin replacement for the hybridoma, myeloma and CHO-K1 cells. Cell growth, cell cycle progression and antibody production was not affected by the substitution. Furthermore, no adaptation procedure was required. © 2004 Kluwer Academic Publishers.
Source Title: Cytotechnology
ISSN: 09209069
DOI: 10.1007/s10616-004-6173-2
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Dec 7, 2018


checked on Nov 28, 2018

Page view(s)

checked on Oct 27, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.