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|Title:||Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures|
Mammalian cell culture
|Citation:||Wong, V.V.T., Ho, K.W., Yap, M.G.S. (2004). Evaluation of insulin-mimetic trace metals as insulin replacements in mammalian cell cultures. Cytotechnology 45 (3) : 107-115. ScholarBank@NUS Repository. https://doi.org/10.1007/s10616-004-6173-2|
|Abstract:||Insulin is involved in a number of cellular functions, including the stimulation of cell growth, cell cycle progression and glucose uptake and is a common protein supplement in serum-free mammalian cell culture media. However, several trace metals have previously been reported to exhibit insulin-like effects on specific cell types. As a step towards developing chemically-defined, protein-free media for mammalian cells, we tested the effectiveness of five trace metals (cadmium, nickel, lithium, vanadium and zinc) as a replacement for insulin. Four cell lines of biotechnological relevance were used, including the hybridoma CRL1606, the myeloma NS0, and the Chinese hamster ovary cell lines CHO-IFN and CHO-K1. Zinc was found to be an effective insulin replacement for the hybridoma, myeloma and CHO-K1 cells. Cell growth, cell cycle progression and antibody production was not affected by the substitution. Furthermore, no adaptation procedure was required. © 2004 Kluwer Academic Publishers.|
|Appears in Collections:||Staff Publications|
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