Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1757-1707.2010.01076.x
Title: Enhanced lipid production in Nannochloropsis sp. using fluorescence-activated cell sorting
Authors: Yen Doan, T.-T.
Obbard, J.P. 
Keywords: Biodiesel
Flow cytometry
Fluorescence-activated cell sorting
Lipids
Nannochloropsis
Sequential sorting
Issue Date: Jun-2011
Citation: Yen Doan, T.-T., Obbard, J.P. (2011-06). Enhanced lipid production in Nannochloropsis sp. using fluorescence-activated cell sorting. GCB Bioenergy 3 (3) : 264-270. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1757-1707.2010.01076.x
Abstract: To advance the utilization of microalgae as a viable feedstock for biodiesel production, the intracellular lipid content of three strains of the marine microalgae Nannochloropsis sp. was enhanced using flow cytometry (FC) coupled with cell sorting. Total lipid content was doubled to 55% (biomass dry weight) in the sorted, daughter cells of Nannochloropsis (strain 47) after consecutive three rounds of cell sorting, and this trait was maintained for approximately 100 subsequent cell generations. In addition, daughter cells had a fatty acid profile similar to that of the parent, wild-type strain. The study demonstrates that FC coupled with cell sorting is a powerful tool for the enhancement of intracellular lipid content in microalgae exploited for biodiesel feedstock. © 2010 Blackwell Publishing Ltd.
Source Title: GCB Bioenergy
URI: http://scholarbank.nus.edu.sg/handle/10635/59038
ISSN: 17571693
DOI: 10.1111/j.1757-1707.2010.01076.x
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