Please use this identifier to cite or link to this item:
|Title:||Direct fermentation of xylan by clostridium strain BOH3 for the production of butanol and hydrogen using optimized culture medium|
Response surface methodology
|Citation:||Rajagopalan, G., He, J., Yang, K.L. (2014-02). Direct fermentation of xylan by clostridium strain BOH3 for the production of butanol and hydrogen using optimized culture medium. Bioresource Technology 154 : 38-43. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2013.11.094|
|Abstract:||Clostridium strain BOH3 is able to utilize 10. g/l of xylan in reinforced clostridial medium (RCM) and produce butanol and hydrogen. However, increasing xylan concentration to 30. g/l does not enhance the production of butanol and hydrogen due to insufficient expression of xylanase enzyme (27.5. U/mg). To enhance the xylanase activity, an optimized culture medium (OCM), which consists of sugarcane bagasse hydrolysate (11.75. g/l), ammonium sulfate (8.92. g/l) and iron (III) chloride (1.45. mM) is designed. In the optimized OCM, Clostridium strain BOH3 expresses more xylanase and shows higher xylanase activity (44.05. ±. 0.25. U/mg) in the OCM. This activity is about 1.6-fold higher than that in the original RCM. Employing OCM as a medium, Clostridium strain BOH3 effectively ferments high concentration (30 and 50. g/l) of xylan and produces 12.05. ±. 0.15 and 14.80. ±. 0.15. g/l of butanol and 1.78. ±. 0.08 and 2.65. ±. 0.15. l/l of hydrogen, respectively. © 2013.|
|Source Title:||Bioresource Technology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 14, 2018
WEB OF SCIENCETM
checked on Oct 30, 2018
checked on Oct 6, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.