Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2010.10.028
Title: A portable generic DNA bioassay system based on in situ oligonucleotide synthesis and hybridization detection
Authors: Wang, C.
Trau, D. 
Keywords: Bio-threat detection
DNA biosensor
DNA synthesis
Homeland security
Issue Date: 15-Jan-2011
Source: Wang, C., Trau, D. (2011-01-15). A portable generic DNA bioassay system based on in situ oligonucleotide synthesis and hybridization detection. Biosensors and Bioelectronics 26 (5) : 2436-2441. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2010.10.028
Abstract: In this study, we present a portable and generic DNA bioassay system based on in situ oligonucleotide synthesis followed by hybridization based detection. The system include two main parts, an oligonucleotide synthesizer and a fluorescence detection system. The oligonucleotide synthesizer is based on microfluidic technology and capable of synthesizing any desired oligonucleotide which can be either used as a primer for PCR based detection (external) or a probe for hybridization based detection (integrated) of a target DNA analyte. The oligonucleotide sequence can be remotely sent to the system. The integrated fluorescence detection system is based on a photodiode to detect Texas Red fluorophore as low as 0.5. fmol. The complete system, integrating the oligonucleotide synthesizer and fluorescence detection system, was successfully used to distinguish DNA from two different bacteria strains. The presented generic portable instrument has the potential to detect any desired DNA target sequence in the field. Potential applications are for homeland security and fast responses to emerging bio-threats. © 2010 Elsevier B.V.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/54723
ISSN: 09565663
DOI: 10.1016/j.bios.2010.10.028
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