Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.colsurfb.2011.09.004
Title: A novel method of AquaporinZ incorporation via binary-lipid Langmuir monolayers
Authors: Sun, G. 
Zhou, H.
Li, Y. 
Jeyaseelan, K.
Armugam, A.
Chung, T.-S. 
Keywords: Aquaporin
Detergent
Langmuir-Blodgett trough
Lipid monolayer
Protein incorporation
Issue Date: 1-Jan-2012
Citation: Sun, G., Zhou, H., Li, Y., Jeyaseelan, K., Armugam, A., Chung, T.-S. (2012-01-01). A novel method of AquaporinZ incorporation via binary-lipid Langmuir monolayers. Colloids and Surfaces B: Biointerfaces 89 (1) : 283-288. ScholarBank@NUS Repository. https://doi.org/10.1016/j.colsurfb.2011.09.004
Abstract: In this work, a new approach of incorporating transmembrane protein AquaporinZ (AqpZ) into a lipid bilayer has been developed with the aid of the Langmuir-Blodgett technique. The binary-lipid monolayer for AqpZ incorporation is composed of (1) gel-phase lipids resistant to detergent dissolution and (2) nickel-chelating lipids that can attach the histidine-tagged AqpZ from the subphase. Upon removal of subphase detergent with BioBeads, the incorporation is achieved by transferring the AqpZ-associated binary-lipid monolayer onto a preformed pure binary-lipid monolayer using the Langmuir-Schaefer deposition method. AFM images show an indication of AqpZ incorporation in the bilayer. Furthermore, it is also shown that BioBeads can remove a significant amount of detergent in the subphase and lipid film integrity is restored after detergent removal. The detergent removal rate is correlated to BioBeads amount and subphase circulation. The new approach of AqpZ reconstitution revealed in this work could potentially be applied in biomimetic membrane formation for water purification applications. © 2011 Elsevier B.V.
Source Title: Colloids and Surfaces B: Biointerfaces
URI: http://scholarbank.nus.edu.sg/handle/10635/54630
ISSN: 09277765
DOI: 10.1016/j.colsurfb.2011.09.004
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