Please use this identifier to cite or link to this item: https://doi.org/10.3109/15419061.2012.665968
Title: 3D coupling of fibronectin fibril arrangement with topology of ventral plasma membrane
Authors: Hirata, H.
Lim, C.T. 
Miyata, H.
Keywords: fibronectin
focal adhesion
integrin
membrane topology
plasma membrane
total internal reflection fluorescence microscopy
Issue Date: Apr-2012
Citation: Hirata, H., Lim, C.T., Miyata, H. (2012-04). 3D coupling of fibronectin fibril arrangement with topology of ventral plasma membrane. Cell Communication and Adhesion 19 (2) : 17-23. ScholarBank@NUS Repository. https://doi.org/10.3109/15419061.2012.665968
Abstract: Interaction of integrins with extracellular matrices is essential for cell adhesion to substrata. Ventral surfaces of fibroblasts adhering to flat substrata are not flat but have uneven 3D topology. However, spatial relationship between the topology of the ventral cell surface and arrangement of extracellular matrix fibrils remains unclear. Here, we report a novel and simple method based on total internal reflection fluorescence microscopy to quantify the distance between the ventral plasma membrane and the glass substratum. We observe that the distance varies from <25 nm at focal adhesions to 40-50 nm at close contacts and >80 nm in other regions. Furthermore, by applying this novel method, we show that fibronectin fibrils are also separated from the substratum in regions where the ventral cell surface-substratum distance is >80 nm. Our results reveal that fibronectin fibrils are not simply adsorbed to the glass substratum but follow the ventral cell surface topology. © 2012 Informa Healthcare USA, Inc.
Source Title: Cell Communication and Adhesion
URI: http://scholarbank.nus.edu.sg/handle/10635/53859
ISSN: 15419061
DOI: 10.3109/15419061.2012.665968
Appears in Collections:Staff Publications

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