Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/53804
Title: STRUCTURE-FUNCTION RELATIONSHIPS AND ACTION MECHANISM OF KRAIT NATRIURETIC PEPTIDE
Authors: SINDHUJA SRIDHARAN
Keywords: Natriuretic peptide, blood pressure, cGMP, vasodilation, krait, venom
Issue Date: 24-Jan-2014
Source: SINDHUJA SRIDHARAN (2014-01-24). STRUCTURE-FUNCTION RELATIONSHIPS AND ACTION MECHANISM OF KRAIT NATRIURETIC PEPTIDE. ScholarBank@NUS Repository.
Abstract: Endogenous natriuretic peptides (NPs) are a class of potent vasoactive hormones, which maintain pressure-volume homeostasis. Snake venom NPs are structurally similar to endogenous NPs with a 17-residue ring but with variable C-terminal extension. In the present study, a unique NP from a krait , KNP, has been studied. Structurally, KNP has a 38-residue long C-terminal tail which has propensity to form a-helix. KNP induced vasorelaxation of aortic strip by a mechanism independent of NP receptors in contrast to canonical NPs. Deletion mutant study has revealed the presence of two functional pharmacophores; KNP ring and putative helix. Ring functions like a classical NP while Helix induces vasorelaxation in NP receptor independent manner like KNP. Thus, the helical segment in KNP?s C-tail seems to confer its bio-activity. KNP showed remarkable resistance (half-life >150 min) to degradation by neural endopeptidase. By specific substitutions, the key residues involved in conferring resistance have been identified.
URI: http://scholarbank.nus.edu.sg/handle/10635/53804
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Sindhuja Sridharan_ final thesis.pdf4.22 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

165
checked on Feb 16, 2018

Download(s)

53
checked on Feb 16, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.