Preparation and application of mixed octadecylsilyl- and (3-(C-methylcalix[4]resorcinarene)-hydroxypropoxy)-propylsilyl-appended silica particles as stationary phase for high-performance liquid chromatography

Abstract

A new type of mixed octadecylsily (ODS)- and (3-(C-methylcalix[4] resorcinarene)-2-hydroxypropoxy)-propylsilyl-appended silica particles (ODS-MCR-HPS) have been prepared and used as stationary phase for high-performance liquid chromatography (HPLC) for the first time. Elemental analysis and Fourier-transform infrared spectroscopy have been applied to characterize the synthetic ODS-MCR-HPS. The new stationary phase contains two types of interaction sites for separation: ODS and MCR. The chromatographic performance of ODS-MCR-HPS was investigated by separating several disubstituted benzenes and some aromatic hydocarbons under both reversed-phase and normal-phase conditions. The results show that ODS-MCR-HPS exhibits excellent selectivity for the separation of positional isomers of disubstituted benzenes and mixtures of some aromatic hydrocarbon compounds due to the cooperative and complementary functioning of the ODS and MCR under different mobile phase conditions. Copyright © Taylor & Francis Group, LLC.