STUDY OF 1-DIMNESIONAL MOVEMENT OF LAMBDA DNA IN NANOCHANNELS

Abstract

This research demonstrates how PDMS casting can be used to investigate fluorescence labelled single Lambda DNA molecules. Analysis of the mean squared displacement data showed a diffusion slope during the first few seconds. At later time?s entropic trapping effect of the DNA in channel leads to an elastic response so that the MSD levels off to a constant value. Additionally, comparisons of diffusions coefficients to the extension of DNA molecules agree with predicted revisiting blob theory.