Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/51951
Title: C. elegans PRDM1/Blimp1 homolog BLMP-1 is a positive regulator of bed-3 transcription
Authors: YANG JIN
Keywords: C. elegans, bed-3, BLMP-1, Blimp1, enhancer element, vulval cells
Issue Date: 22-Jan-2014
Source: YANG JIN (2014-01-22). C. elegans PRDM1/Blimp1 homolog BLMP-1 is a positive regulator of bed-3 transcription. ScholarBank@NUS Repository.
Abstract: C. elegans is a model organism which can be used as an effective tool to investigate conserved mechanisms regulating development and gene expression. Blimp1 is the master regulator for B lymphocytes terminal differentiation in humans. Blimp1 can activate or repress transcription. However, the activator function of Blimp1 is not well understood. Here, we focused on BLMP-1, the C. elegans ortholog of Blimp1. Previously a putative BLMP-1 binding site was identified in intron 3 of bed-3, a gene regulating molting and cell division. Furthermore, the binding site mapped to within an enhancer element required for bed-3 expression. Here, using EMSA and in vivo enhancer assays, we provide evidence that BLMP-1 functions as a transcriptional activator of bed-3 by directly binding to the enhancer element. The role of BLMP-1 as a transcriptional activator in C. elegans may help us better understand the complicated functions of Blimp1 in humans.
URI: http://scholarbank.nus.edu.sg/handle/10635/51951
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