Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.msec.2010.06.004
Title: Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering
Authors: Ghasemi-Mobarakeh, L.
Prabhakaran, M.P. 
Morshed, M.
Nasr-Esfahani, M.H.
Ramakrishna, S. 
Keywords: Alkaline hydrolysis
Matrigel
Nanofibrous scaffolds
Nerve tissue engineering
PCL
Issue Date: 12-Oct-2010
Source: Ghasemi-Mobarakeh, L., Prabhakaran, M.P., Morshed, M., Nasr-Esfahani, M.H., Ramakrishna, S. (2010-10-12). Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering. Materials Science and Engineering C 30 (8) : 1129-1136. ScholarBank@NUS Repository. https://doi.org/10.1016/j.msec.2010.06.004
Abstract: Surface properties of scaffolds such as hydrophilicity and the presence of functional groups on the surface of scaffolds play a key role in cell adhesion, proliferation and migration. Different modification methods for hydrophilicity improvement and introduction of functional groups on the surface of scaffolds have been carried out on synthetic biodegradable polymers, for tissue engineering applications. In this study, alkaline hydrolysis of poly (ε-caprolactone) (PCL) nanofibrous scaffolds was carried out for different time periods (1 h, 4 h and 12 h) to increase the hydrophilicity of the scaffolds. The formation of reactive groups resulting from alkaline hydrolysis provides opportunities for further surface functionalization of PCL nanofibrous scaffolds. Matrigel was attached covalently on the surface of an optimized 4 h hydrolyzed PCL nanofibrous scaffolds and additionally the fabrication of blended PCL/matrigel nanofibrous scaffolds was carried out. Chemical and mechanical characterization of nanofibrous scaffolds were evaluated using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, contact angle, scanning electron microscopy (SEM) and tensile measurement. In vitro cell adhesion and proliferation study was carried out after seeding nerve precursor cells (NPCs) on different scaffolds. Results of cell proliferation assay and SEM studies showed that the covalently functionalized PCL/matrigel nanofibrous scaffolds promote the proliferation and neurite outgrowth of NPCs compared to PCL and hydrolyzed PCL nanofibrous scaffolds, providing suitable substrates for nerve tissue engineering. © 2010 Elsevier B.V.
Source Title: Materials Science and Engineering C
URI: http://scholarbank.nus.edu.sg/handle/10635/51342
ISSN: 09284931
DOI: 10.1016/j.msec.2010.06.004
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

81
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

70
checked on Nov 15, 2017

Page view(s)

35
checked on Dec 16, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.