Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49347
Title: MOLECULAR STUDIES ON THE RESPONSE OF PERIODONTAL LIGAMENT CELLS TO MECHANICAL STRAIN IN VITRO
Authors: AARTHI SAMINATHAN
Keywords: Periodontal Ligament, Hydrogels, Tissue engineering, Mechanical stress
Issue Date: 22-Aug-2013
Source: AARTHI SAMINATHAN (2013-08-22). MOLECULAR STUDIES ON THE RESPONSE OF PERIODONTAL LIGAMENT CELLS TO MECHANICAL STRAIN IN VITRO. ScholarBank@NUS Repository.
Abstract: Periodontal Ligament functions in a mechanically active environment. Most in-vitro studies used the traditional 2-D culture systems limiting the knowledge about the cells behaviour in-vivo. To overcome this limitation, we have engineered and characterized 3-dimensional constructs of the PDL by seeding cells into hydrogel matrices capable of being mechanically-deformed. Subjecting these constructs to biaxial cyclic strain of 12% did not have a significant effect on the viability of cells. Twelve of 18 extracellular genes analysed were expressed and upregulated at some experimental time point as was the synthesis of MMPs and TIMP-1. Cyclic compressive strain of 0.5lbs significantly increased the number of non-viable cells. Twelve of the twenty extracellular matrix genes analysed and fifteen apoptotic genes were expressed with significant synthesis of matrix proteins. These 3-dimensional culture systems are suitable as transitional in-vitro model for studying cell-cell and cell-matrix interactions in tooth support and investigating the pathogenesis of periodontal diseases.
URI: http://scholarbank.nus.edu.sg/handle/10635/49347
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
SaminathanA.pdf4.39 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

193
checked on Dec 18, 2017

Download(s)

33
checked on Dec 18, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.