Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/49283
Title: HEPATITIS B PREGENOMICS RNA SPLICING AND ITS ROLE IN VIRAL PERSISTANCE
Authors: LEE GUAN HUEI
Keywords: hepatitis B, splice, replication, seroconversion, core, siRNA
Issue Date: 31-Jan-2013
Source: LEE GUAN HUEI (2013-01-31). HEPATITIS B PREGENOMICS RNA SPLICING AND ITS ROLE IN VIRAL PERSISTANCE. ScholarBank@NUS Repository.
Abstract: Hepatitis B hijacks the human splicing mechanisms to generate the HBV splice variants. The work in this thesis focused on finding the biological roles of HBV RNA splicing. We found that HBV splice variants were common and abundant in chronic infection, and that the levels rise during the immune clearance phase and peaked just before HBeAg seroconversion. This can be linked to the inhibition of the HBV polymerase function, possibly mediated through the interferons. Our co-transfection experiments showed that the single-spliced defective HBV genome enhanced replication of wild-type HBV DNA. The expression plasmid transfection experiments showed that core protein is the rate-limiting factor of the viral replication, and that spliced RNA may serve as dedicated core mRNA to support viral replication. Small interfering RNA targeting the splice donor and acceptor sites, and the PRE region were found to be effective in suppressing the viral expression and replication.
URI: http://scholarbank.nus.edu.sg/handle/10635/49283
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
LeeGH.pdf9.87 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

82
checked on Dec 11, 2017

Download(s)

76
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.