Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/48676
Title: Uni-axial Stretching Generated Poly (E-caprolactone) Film for Human Vascular Tissue Engineering Application
Authors: WANG ZUYONG
Keywords: Vascular Tissue Engineering, Polycaprolactone, Mesenchymal Stem Cell, Geometric Cues, Uni-axial Stretching, Femtosecond Laser Process
Issue Date: 14-Aug-2013
Source: WANG ZUYONG (2013-08-14). Uni-axial Stretching Generated Poly (E-caprolactone) Film for Human Vascular Tissue Engineering Application. ScholarBank@NUS Repository.
Abstract: Poor scaffold function could impair vascular tissue regeneration, limiting the clinical translation of tissue-engineered vascular grafts. In this work, poly(E-caprolactone) (PCL) films with anisotropic geometries and micropore patterns were developed and fabricated, to mimic the extracellular matrices (ECM) for the complex architecture reconstruction of blood vessels. Uniaxial stretching induced three-dimensional orientated ridge/groove arrays on PCL films with enhanced mechanical properties. Mesenchymal stem cells (MSCs) grew and aligned well on the PCL films with up-regulated expression of contractile smooth muscle cell genes. In addition, the PCL films exhibited inherent stability in an erosive environment, thereby better retaining the anisotropic geometries. PCL films with micropore patterns could be achieved via femtosecond laser drilling, which mimicked the vascular basement membrane for nutrient permeability, MSCs alignment on one surface of the films, and rapid endothelialisation on the opposite surface of the films. In short, this thesis explores the use of anisotropic geometries and micropore patterns for the generation of multi-functional film scaffolds that are capable of mimicking ECM for facilitating vascular tissue regeneration.
URI: http://scholarbank.nus.edu.sg/handle/10635/48676
Appears in Collections:Ph.D Theses (Open)

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