Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/48646
Title: SEGMENTAL RENAL GENE EXPRESSION OF DRUG TRANSPORTERS IN A RAT MODEL OF TYPE II DIABETES
Authors: YEE JIE YIN (YU JIEYIN)
Keywords: qPCR, LCM, drug transporters, diabetes, rats
Issue Date: 27-Jun-2013
Source: YEE JIE YIN (YU JIEYIN) (2013-06-27). SEGMENTAL RENAL GENE EXPRESSION OF DRUG TRANSPORTERS IN A RAT MODEL OF TYPE II DIABETES. ScholarBank@NUS Repository.
Abstract: This study aims to examine changes in gene expression levels of drug transporters located on rodent renal proximal tubules in progressive stages of diabetes. A combination of high-fat diet and single dose streptozotocin (35mg/kg) was used in the induction of diabetes in male Wistar rats. Previously reported drug transporters which were localised on rat kidneys were included in current study. Laser capture microdissection was used in the collection of rat renal proximal tubular cells and quantitative real-time polymerase chain reaction was applied for the analysis of changes in drug transporters¿ gene expression levels. Clinical diabetic nephropathy was not observed in renal functions and histological analysis. Transforming growth factor-beta was observed to be higher in renal proximal tubular cells of diabetic rats, suggesting tubular injury may occur before glomerular damage. Changes in gene expression levels of transporters during maturation and progressive diabetes were observed in several drug transporters.
URI: http://scholarbank.nus.edu.sg/handle/10635/48646
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
YeeJY.pdf2.09 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

92
checked on Dec 1, 2017

Download(s)

34
checked on Dec 1, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.