Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/48523
Title: REPRESENTATION OF OLFACTORY CUES IN THE ZEBRAFISH HABENULA
Authors: KRISHNAN SEETHA LAKSHMI
Keywords: Zebrafish, Habenula, Olfaction, Volume Imaging, GCaMP3, Odor-Evoked Behavior
Issue Date: 24-Jul-2013
Source: KRISHNAN SEETHA LAKSHMI (2013-07-24). REPRESENTATION OF OLFACTORY CUES IN THE ZEBRAFISH HABENULA. ScholarBank@NUS Repository.
Abstract: The habenula consists of an evolutionarily conserved set of nuclei that regulate the release of monoamines and neuropeptides. In lower vertebrates, the habenula receives direct input from the olfactory system, but its function in olfactory-guided behaviour is unknown. To investigate this, we imaged neural activity in larval zebrafish expressing GCaMP3 throughout the habenula. Activity in several hundred neurons per fish was recorded each second using an EMCCD camera and fast focusing, enabling creation of three-dimensional maps of odor-evoked activity. Odors that activate the medio-dorsal olfactory bulb, including a bile salt, a diamine and a polysaccharide, asymmetrically activate the dorsal right habenula. In contrast, amino acids activating the ventral olfactory bulb trigger a response primarily in the ventral habenula. Lesioning the dorsal right habenula alters preference in a behavioral assay of concentration-dependent odor choice. These data establish that odors activate the habenula in distinct spatio-temporal patterns that influence odor-evoked behaviors.
URI: http://scholarbank.nus.edu.sg/handle/10635/48523
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
01Chap.pdf3.67 MBAdobe PDF

OPEN

NoneView/Download
02Chap.pdf8.21 MBAdobe PDF

OPEN

NoneView/Download
03Chap.pdf3.73 MBAdobe PDF

OPEN

NoneView/Download
04Chap.pdf8.57 MBAdobe PDF

OPEN

NoneView/Download
05Chap.pdf5.18 MBAdobe PDF

OPEN

NoneView/Download
06Chap.pdf1.3 MBAdobe PDF

OPEN

NoneView/Download
07Chap.pdf187.91 kBAdobe PDF

OPEN

NoneView/Download

Page view(s)

126
checked on Dec 11, 2017

Download(s)

144
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.