Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbm.a.30622
Title: Enterococcus faecalis-mediated biomineralized biofilm formation on root canal dentine in vitro
Authors: Kishen, A. 
George, S.
Kumar, R.
Keywords: Apatite
Bacteria
Biofilm
Calcification
Dentine
Issue Date: 2006
Source: Kishen, A., George, S., Kumar, R. (2006). Enterococcus faecalis-mediated biomineralized biofilm formation on root canal dentine in vitro. Journal of Biomedical Materials Research - Part A 77 (2) : 406-415. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.a.30622
Abstract: Enterococcus faecalis is the most predominant bacteria in teeth with failed root canal therapy and is found to survive harsh conditions prevailing in the root canals of endodontically treated teeth. This study aims to investigate the interaction between E. faecalis and root canal dentine substrate. Towards this end, tooth specimens were prepared and divided into two groups. The tooth specimens in group 1 were incubated with E. faecalis for periods of 2-, 4-, and 6-week intervals and the chemical composition of the biofilm was determined using X-ray diffraction and Fourier transform infrared (FTIR) spectroscopy. The tooth specimens in group 2 were incubated with E. faecalis for a period of 6 weeks and the topography and ultrastructure of the biofilm were examined using scanning electron microscopy (SEM), light microscopy, and laser confocal scanning microscopy. The sediments formed from the bacterial interaction on the dentine (in group 1) were also examined by SEM and FTIR. These experiments highlighted different stages in the interaction of E. faecalis with root canal dentine. Further, a bacterial-induced apatite reprecipitation on mature biofilm was also observed. This ability of E. faecalis to form such calcified biofilm on root canal dentine may be a factor that contributes to their persistence after endodontic treatment. © 2006 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/46878
ISSN: 15493296
DOI: 10.1002/jbm.a.30622
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