Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.joen.2007.07.035
Title: Effect of Tissue Fluids on Hydrophobicity and Adherence of Enterococcus faecalis to Dentin
Authors: George, S.
Kishen, A. 
Keywords: Adherence
dentin
Enterococcus faecalis
hydrophobicity
saliva
serum
Issue Date: 2007
Source: George, S., Kishen, A. (2007). Effect of Tissue Fluids on Hydrophobicity and Adherence of Enterococcus faecalis to Dentin. Journal of Endodontics 33 (12) : 1421-1425. ScholarBank@NUS Repository. https://doi.org/10.1016/j.joen.2007.07.035
Abstract: This in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of Enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adherence of E. faecalis to dentin. The ability to bind to a hydrocarbon phase (xylene) was used as an index of relative hydrophobicity of cells. Fluorescent microscopy-based technique was used to assay the bacterial adherence to dentin. Results showed that bacteria involved in the primary stage of oral biofilm formation such as Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis are relatively more hydrophobic than E. faecalis. The hydrophobicity of E. faecalis was significantly increased during starvation and biofilm mode of growth (p < .05). The adherence of E. faecalis to dentin was appreciably increased after starvation and when dentin was conditioned with saliva. It was observed that surface conditioning of dentin with saliva and starvation can enhance the adherence of E. faecalis to dentin. The findings from this study indicated that the coronal leakage of saliva and the physiologic state of microbes might play an important role in the adherence and biofilm formation of bacteria to root canal dentin. © 2007 American Association of Endodontists.
Source Title: Journal of Endodontics
URI: http://scholarbank.nus.edu.sg/handle/10635/46855
ISSN: 00992399
DOI: 10.1016/j.joen.2007.07.035
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

15
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

12
checked on Nov 21, 2017

Page view(s)

77
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.