Please use this identifier to cite or link to this item:
|Title:||Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma|
|Authors:||Sung, W.-K. |
|Citation:||Sung, W.-K., Zheng, H., Li, S., Chen, R., Liu, X., Li, Y., Lee, N.P., Lee, W.H., Ariyaratne, P.N., Tennakoon, C., Mulawadi, F.H., Wong, K.F., Liu, A.M., Poon, R.T., Fan, S.T., Chan, K.L., Gong, Z., Hu, Y., Lin, Z., Wang, G., Zhang, Q., Barber, T.D., Chou, W.-C., Aggarwal, A., Hao, K., Zhou, W., Zhang, C., Hardwick, J., Buser, C., Xu, J., Kan, Z., Dai, H., Mao, M., Reinhard, C., Wang, J., Luk, J.M. (2012). Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma. Nature Genetics 44 (7) : 765-769. ScholarBank@NUS Repository. https://doi.org/10.1038/ng.2295|
|Abstract:||To survey hepatitis B virus (HBV) integration in liver cancer genomes, we conducted massively parallel sequencing of 81 HBV-positive and 7 HBV-negative hepatocellular carcinomas (HCCs) and adjacent normal tissues. We found that HBV integration is observed more frequently in the tumors (86.4%) than in adjacent liver tissues (30.7%). Copy-number variations (CNVs) were significantly increased at HBV breakpoint locations where chromosomal instability was likely induced. Approximately 40% of HBV breakpoints within the HBV genome were located within a 1,800-bp region where the viral enhancer, X gene and core gene are located. We also identified recurrent HBV integration events (in ≥4 HCCs) that were validated by RNA sequencing (RNA-seq) and Sanger sequencing at the known and putative cancer-related TERT, MLL4 and CCNE1 genes, which showed upregulated gene expression in tumor versus normal tissue. We also report evidence that suggests that the number of HBV integrations is associated with patient survival. © 2012 Nature America, Inc. All rights reserved.|
|Source Title:||Nature Genetics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on Apr 30, 2018
checked on Feb 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.