Local Induction of Heat Shock Proteins using Magnetic Fluid hyperthermia for Ocular-Neuroprotection in Glaucoma

Abstract

Recently, ocular neuroprotection induced by heat shock proteins (HSPs)72 has been paid great attention as an effective treatment modality for glaucoma. However, improvement of HSPs72 induction rate and minimization of healthy cell death rate during induction of HSPs72 have been raised as critical challenges to realize the ocular neuroprotection modality in clinics. In this study, AC heating stress controllable magnetic fluid hyperthermia (MFH) using superparamagnetic nanoparticles (SPNPs) was designed to effectively improve the induction efficiency of HSPs72 and demonstrated to be promising for high efficient local induction of HSPs72 in retinal ganglion cells (RGCs). For this work, a high performance biocompatible SPNP agent was developed to successfully control the AC heating stress in RGCs. It was verified that the physiological correlation between the HSPs72 induction process and the response of RGCs to the controlled AC heating stress was mainly responsible for the high efficient induction of HSPs72.