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|Title:||Effect of overexpression of Bcl-2 on cellular oxidative damage, nitric oxide production, antioxidant defenses, and the proteasome|
Nitric oxide synthase, Proteasome
Reactive nitrogen species
|Citation:||Lee, M., Hyun, D.-H., Marshall, K.-A., Jenner, P., Halliwell, B., Ellerby, L.M., Bredesen, D.E. (2001). Effect of overexpression of Bcl-2 on cellular oxidative damage, nitric oxide production, antioxidant defenses, and the proteasome. Free Radical Biology and Medicine 31 (12) : 1550-1559. ScholarBank@NUS Repository. https://doi.org/10.1016/S0891-5849(01)00633-5|
|Abstract:||Bcl-2 is a gene family involved in the suppression of apoptosis in response to a wide range of cellular insults. Multiple papers have suggested a link between Bcl-2 and oxidative damage/antioxidant protection. We therefore examined parameters of antioxidant defense and oxidative damage in two different cell lines, NT-2/D1 (NT-2) and SK-N-MC, overexpressing Bcl-2 as compared with vector-only controls. Bcl-2 transfectants of both cell lines were more resistant to H2O2 and showed increases in GSH level and Cu/Zn-superoxide dismutase (SOD1) activity, but not in Mn-superoxide dismutase, glutathione peroxidase, or glutathione reductase activities. Catalase activity was increased in SK-N-MC cells. Overexpression of Bcl-2 did not significantly decrease levels of oxidative DNA damage (measured as 8-hydroxyguanine) or lipid peroxidation, but it decreased levels of 3-nitrotyrosine in both cell lines and protein carbonyls in SK-N-MC cells only. It also increased proteasome activity in both cell lines. We conclude that Bcl-2 raises cellular antioxidant defense status, but this is not necessarily reflected in decreased levels of oxidative damage to DNA and lipids. The ability of Bcl-2 overexpression to decrease 3-nitrotyrosine levels suggests that it may decrease formation of peroxynitrite or other reactive nitrogen species; this was confirmed as decreased production of NO2-/NO3- in the transfected cells and a fall in the level of nNOS protein. © 2001 Elsevier Science Inc.|
|Source Title:||Free Radical Biology and Medicine|
|Appears in Collections:||Staff Publications|
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