Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2004.05.076
Title: Molecular cloning and biochemical characterization of Candida albicans acyl-CoA:sterol acyltransferase, a potential target of antifungal agents
Authors: Kim, K.-Y.
Shin, Y.-K.
Paik, Y.-K.
Park, J.-C.
Kim, J.-H.
Yang, H. 
Han, D.-M.
Keywords: Acyl-coenzyme A:sterol acyltransferase
Antifungal agent
Candida albicans
Ergosterol
HWY-289
Sterol ester
Issue Date: 2004
Source: Kim, K.-Y., Shin, Y.-K., Paik, Y.-K., Park, J.-C., Kim, J.-H., Yang, H., Han, D.-M. (2004). Molecular cloning and biochemical characterization of Candida albicans acyl-CoA:sterol acyltransferase, a potential target of antifungal agents. Biochemical and Biophysical Research Communications 319 (3) : 911-919. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2004.05.076
Abstract: To determine whether Candida albicans acyl CoA:sterol acyltransferase (ASAT) can be a potential target enzyme for the protoberberine derivative (HWY-289), we have isolated a gene encoding Ca-ASAT and examined inhibitory effects of HWY-289 on the overexpressed Ca-ASAT. HWY-289 specifically inhibits Ca-ASAT in a non-competitive manner in vitro (IC50 [9.2μM], K i [5.15μM]). The cloned CaARE2 gene (1830 nucleotides [nt]) encodes active Ca-ASAT protein that exhibits a calculated molecular mass of 71.3kDa. The amino acid sequence of CaAre2p is 33.4% and 35.1% identical to those of Saccharomyces cerevisiae ScAre1p and ScAre2p homologues, respectively. Recombinant and endogenous Ca-ASAT displayed identical patterns of inhibition upon exposure to HWY-289 and a preference for cholesterol and oleoyl-CoA as substrates. Northern blot analysis showed that CaARE2 was activated by HWY-289, but not by CI-976 (a human acyl-coenzyme A:cholesterol acyltransferase inhibitor), in a dose-dependent manner (up to 5mg/L), suggesting different selectivities of action between HWY-289 and CI-976 on Ca-ASAT activity. © 2004 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/38251
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2004.05.076
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