Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/36553
Title: ASSOCIATION OF PLACENTAL SYNCYTIOTROPHOBLAST MEMBRANE MICRO-PARTICLES (STBM) WITH MATERNAL IMMUNE RESPONSE IN ADVERSE PREGNANCY OUTCOMES
Authors: SONIA BAIG
Keywords: Placenta, microvesicles, immunomodulation, dendritic cells, recurrent miscarriages, preeclampsia
Issue Date: 23-Aug-2012
Source: SONIA BAIG (2012-08-23). ASSOCIATION OF PLACENTAL SYNCYTIOTROPHOBLAST MEMBRANE MICRO-PARTICLES (STBM) WITH MATERNAL IMMUNE RESPONSE IN ADVERSE PREGNANCY OUTCOMES. ScholarBank@NUS Repository.
Abstract: Problem: Placental syncytiotrophoblast microvesicles (STBM) may modify maternal immune response. We therefore sought to (1) evaluate the immunogenicity of STBM in women with recurrent miscarriages (RM) and preeclampsia (2) determine the bioactive contents of STBM (3) phenotype maternal decidual immune cells. Methods: STBM from women with adverse or normal pregnancy, isolated from placental explant culture supernatant, were used in dendritic cell (DC) stimulation assays followed by cytokine ELISA and flow cytometry. STBM bioactive contents were investigated by ESI LC-MS/MS of proteins and lipids. Decidual dendritic cell subsets were studied by a 13-colour flow cytometry. Results: Pathologic STBM are more immunogenic producing significantly increased level of pro-inflammatory cytokines such as IL-6 and TNF-a from DC. STBM proteins & lipids are differentially expressed in pathologic pregnancies. Conclusion: Our findings lend support to the emerging concept of immune-dysregulation in preeclampsia and RM. This has implications in development of novel diagnostic and therapeutic approaches.
URI: http://scholarbank.nus.edu.sg/handle/10635/36553
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
BaigS.pdf13.35 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

189
checked on Dec 11, 2017

Download(s)

60
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.