Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34935
Title: C-terminal attachment of ligands to proteins for immobilization onto a support
Authors: YAO, SHAO QIN 
TAN, LAY PHENG 
LUE, YEE PENG RINA 
Issue Date: 12-Jan-2006
Source: YAO, SHAO QIN,TAN, LAY PHENG,LUE, YEE PENG RINA (2006-01-12). C-terminal attachment of ligands to proteins for immobilization onto a support. ScholarBank@NUS Repository.
Abstract: The present invention provides methods of immobilizing proteins onto a support, using a cellular expression system or a cell-free expression system, to attach a ligand to the C-terminus of the protein, by an intein-mediated or a puromycin-mediated approach. A method for improving the efficiency of intein-mediated ligand attachment is also provided. The methods of the present invention are useful in the preparation of protein microarrays.
URI: http://scholarbank.nus.edu.sg/handle/10635/34935
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